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DNA Sequencing

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Co-Resource Directors: Terri Goss Kinzy, Ph.D. and Lee Ann Schein, Ph.D.

The DNA Sequencing Shared Resource of The Cancer Institute of New Jersey (CINJ) provides a centralized, high quality, cost-effective facility for DNA sequencing, analysis, and synthesis for use by CINJ members.  The shared resource represents an expansion of the original Robert Wood Johnson Medical School (RWJMS) facility.  This facility was established in 1985 with funds from the New Jersey Commission on Science and Technology and RWJMS. 

The specific objectives of this shared resource are to:

  1. Provide fast and accurate DNA sequencing, single nucleotide polymorphism (SNP) studies, and fragment analysis;
  2. Provide centralized access to high throughput Real-Time PCR and all associated  applications:  TaqMan®® Assays, Allelic Discrimination and SNP Analysis, Gene Expression, Quantitative PCR, mRNA Expression and Mutation Detection;
  3. Assist users in the development of individual assays and applications for genomic analysis and SNP experiments;
  4. Consult on the design and use of oligonucleotides for molecular experimentation;
  5. Eliminate the need for duplicate equipment and software purchases by multiple CINJ member laboratories;
  6. Provide centralized, discounted access to synthetic oligonucleotides to CINJ members for use in DNA sequencing, polymerase chain reactions, site-directed mutagenesis, hybridization, and structural studies, synthetic RNAs and modified fluorescent oligonucleotides for Loss of Heterozygosity and Microsatellite Instability (LOH and MIN) fragment analysis, and dual labeled probes for Real-Time PCR experiments and SNP studies;
  7. Supply selected Applied Biosystems consumables and reagents to the CINJ research community at a significant discount; and
  8. Provide and sponsor training and technology seminars to enhance the performance of DNA analysis methods by CINJ members.

The DNA Sequencing Core Facility runs two, state-of-the-art, automated 16-capillary electrophoresis ABI 3100 DNA sequencers. The DNA Facility also has all of the equipment and software necessary for Loss of Heterozygosity and Microsatellite Instability (LOH and MIN) fragment analysis and Single Nucleotide Polymorphism (SNP) studies.  In addition, the DNA Core Facility operates an ABI 7900HT Real-Time PCR system.  The DNA Sequencing Shared Resource provides high quality DNA services for members of CINJ, thus freeing them to focus on the application of these technologies in their research.  By serving as a centralized location to order synthetic oligonucleotides, CINJ members can apply DNA sequencing, polymerase chain reactions, site-directed mutagenesis, hybridization, and structural analyses to their studies at a significant cost discount over what individual laboratories could obtain.  The technical support provided by the staff for designing experiments and assay conditions as well as specialty oligonucleotides greatly speeds the application of these methods to cancer research.  This service is especially useful to investigators beginning to use new techniques.

Fees and additional information about this resource can be found at: http://www2.umdnj.edu/dnalbweb